Kinetic data for SABIO kinetic law id 52092

General information
Organism Capsicum annuum
Strain var. Nockwang
Tissue -
EC Class 4.1.1.28
SABIO reaction id 1065
Variant wildtype His-tagged
Recombinant expressed in Escherichia coli BL21
Experiment Type in vitro
Pathways Biosynthesis of secondary metabolites
Indole alkaloid biosynthesis
Metabolic pathways
Tryptophan metabolism
Event Description -
Substrates
name location comment
L-Tryptophan - -
Products
name location comment
CO2 - -
Tryptamine - -
Modifiers
name location effect comment protein complex
aromatic-L-amino-acid decarboxylase(Enzyme) - Modifier-Catalyst purified C0LIF1;
Enzyme (protein data)
UniProtKB_AC name mol. weight (kDa) deviation (kDa)
subunit - - - -
complex - - 56.0 -
Kinetic Law
type formula annotation
Michaelis-Menten Vmax*S/(Km+S) SBO:0000029
Parameter
name type species start val. end val. deviat. unit comment
S concentration L-Tryptophan 2.0 - - mM -
Km Km L-Tryptophan 0.729 - - mM -
Vmax Vmax - 310.0 - - pkatal/mg -
Experimental conditions
start value end value unit
temperature 37.0 - °C
pH 7.5 - -
buffer 10 mM TrisHCl, 0.1 mM pyridoxal-5'-phosphate
General comment
Fruits infected with anthracnose fungus
Reference
title author year journal volume pages PubMed
Induction of serotonin biosynthesis is uncoupled from the coordinated induction of tryptophan biosynthesis in pepper fruits (Capsicum annuum) upon pathogen infection Park S, Kang K, Lee K, Choi D, Kim YS, Back K 2009 Planta 230 1197-206 19760262