Enzyme Details
EC Classification
3.4.24.27
Enzyme name
(recommended)
thermolysin
Alternative names
Bacillus thermoproteolyticus neutral proteinase
thermoase
thermoase Y10
TLN
Classification
Hydrolases
Acting on peptide bonds (peptide hydrolases)
Metalloendopeptidases
Catalyses reactions
H2O + Z-Phe-Leu-Ala-OH <-> Leu-Ala-OH + Benzyloxycarbonyl-Phe
H2O + Benzyloxycarbonyl-Gly-Leu-NH2 <-> Leucinamide + Benzyloxycarbonyl-Gly
H2O + Benzyloxycarbonyl-Gly-Leu-Gly <-> Benzyloxycarbonyl-Gly + L-Leucylglycine
H2O + Benzyloxycarbonyl-Gly-Leu-Ala <-> Leu-Ala + Benzyloxycarbonyl-Gly
H2O + Benzyloxycarbonyl-Gly-Leu-D-Ala <-> Benzyloxycarbonyl-Gly + Leu-D-Ala
H2O + Benzyloxycarbonyl-Gly-Leu-Leu <-> Benzyloxycarbonyl-Gly + Leu-Leu
H2O + Benzyloxycarbonyl-Gly-Leu-Phe <-> Leu-Phe + Benzyloxycarbonyl-Gly
H2O + Benzyloxycarbonyl-Gly-Leu-Gly-Gly <-> Leu-Gly-Gly + Benzyloxycarbonyl-Gly
H2O + Benzyloxycarbonyl-Gly-Leu-Gly-Ala <-> Benzyloxycarbonyl-Gly + Leu-Gly-Ala
H2O + Benzyloxycarbonyl-Gly-Leu-Gly-D-Ala <-> Benzyloxycarbonyl-Gly + Leu-Gly-D-Ala
H2O + Benzyloxycarbonyl-Gly-Leu-Gly-Phe <-> Benzyloxycarbonyl-Gly + Leu-Gly-Phe
H2O + Benzyloxycarbonyl-Ala-Leu-Ala <-> Leu-Ala + Benzyloxycarbonyl-Ala
H2O + Benzyloxycarbonyl-Phe-Leu-Ala <-> Leu-Ala + Benzyloxycarbonyl-Phe
H2O + Benzyloxycarbonyl-Gly-Gly-Leu-Ala <-> Leu-Ala + Benzyloxycarbonyl-Gly-Gly
H2O + Benzyloxycarbonyl-Ala-Gly-Leu-Ala <-> Leu-Ala + Benzyloxycarbonyl-Ala-Gly
H2O + Benzyloxycarbonyl-D-Ala-Gly-Leu-Ala <-> Leu-Ala + Benzyloxycarbonyl-D-Ala-Gly
H2O + Benzyloxycarbonyl-Phe-Gly-Leu-Ala <-> Leu-Ala + Benzyloxycarbonyl-Phe-Gly
H2O + Phe-Gly-Leu-Ala <-> Phe-Gly + Leu-Ala
H2O + Benzyloxycarbonyl-Ala-Gly-Gly-Leu-Ala <-> Leu-Ala + Benzyloxycarbonyl-Ala-Gly-Gly
H2O + Benzyloxycarbonyl-D-Ala-Gly-Gly-Leu-Ala <-> Leu-Ala + Benzyloxycarbonyl-D-Ala-Gly-Gly
H2O + Benzyloxycarbonyl-Gly-Ser-NH2 <-> Benzyloxycarbonyl-Gly + Serinamide
H2O + Benzyloxycarbonyl-Gly-Ala-NH2 <-> L-Alaninamide + Benzyloxycarbonyl-Gly
H2O + Benzyloxycarbonyl-Gly-Val-NH2 <-> Benzyloxycarbonyl-Gly + Valinamide
H2O + Benzyloxycarbonyl-Gly-Ile-NH2 <-> Benzyloxycarbonyl-Gly + Isoleucinamide
H2O + Benzyloxycarbonyl-Gly-Phe-NH2 <-> L-Phenylalaninamide + Benzyloxycarbonyl-Gly
H2O + Benzyloxycarbonyl-Gly-Tyr-NH2 <-> Benzyloxycarbonyl-Gly + Tyrosinamide
H2O + Benzyloxycarbonyl-Tyr-Gly-NH2 <-> Glycine amide + Benzyloxycarbonyl-Tyr
H2O + Benzyloxycarbonyl-Tyr-Ser-NH2 <-> Serinamide + Benzyloxycarbonyl-Tyr
H2O + Benzyloxycarbonyl-Phe-Gly-Ala <-> Gly-Ala + Benzyloxycarbonyl-Phe
H2O + Benzyloxycarbonyl-Phe-Ser-Ala <-> Benzyloxycarbonyl-Phe + Ser-Ala
H2O + Benzyloxycarbonyl-Phe-Ala-Ala <-> Ala-Ala + Benzyloxycarbonyl-Phe
H2O + Benzyloxycarbonyl-Phe-Val-Ala <-> Benzyloxycarbonyl-Phe + Val-Ala
H2O + Benzyloxycarbonyl-Phe-Ile-Ala <-> Benzyloxycarbonyl-Phe + Ile-Ala
H2O + Benzyloxycarbonyl-Phe-Phe-Ala <-> Phe-Ala + Benzyloxycarbonyl-Phe
H2O + Benzyloxycarbonyl-Phe-Tyr-Ala <-> Tyr-Ala + Benzyloxycarbonyl-Phe
H2O + Benzyloxycarbonyl-Phe-Gly-Gly-Leu-Ala <-> Leu-Ala + Benzyloxycarbonyl-Phe-Gly-Gly
H2O + Phe-Gly-Gly-Leu-Ala <-> Phe-Gly-Gly + Leu-Ala
H2O + Benzyloxycarbonyl-Gly-Pro-Leu-Gly <-> Z-Gly-Pro + L-Leucylglycine
H2O + Z-Gly-Pro-Leu-Gly-Pro <-> Z-Gly-Pro + Leu-Gly-Pro
External links
Expasy
KEGG
BRENDA
IntEnz (EBI)
IUBMB
Reactome
MetaCrop
View all entries for EC Class:
3.4.24.27
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