Enzyme Details

EC Classification

3.4.22.69

Enzyme name
(recommended)

SARS coronavirus main proteinase

Alternative names

3cLpro
3C-like protease
coronavirus 3C-like protease
Mpro
SARS 3C-like protease
SARS coronavirus 3CL protease
SARS coronavirus main peptidase
SARS coronavirus main protease
SARS-CoV 3CLpro enzyme
SARS-CoV main protease
SARS-CoV Mpro
severe acute respiratory syndrome coronavirus main protease

Classification

Hydrolases

Acting on peptide bonds (peptide hydrolases)

Cysteine endopeptidases

Catalyses reactions

H2O + Dabcyl-KTSAVLQSGFRKM-Edans <-> SGFRKM-Edans + Dabcyl-KTSAVLQ

H2O + Thr-Ser-Ala-Val-Leu-Gln-4-nitroanilide <-> 4-Nitroaniline + Thr-Ser-Ala-Val-Leu-Gln

H2O + Dabcyl-KTSAVLQSGFRKME-Edans <-> Dabcyl-KTSAVLQ + SGFRKME-Edans

2 3CLpro <-> 3CLpro-dimer

H2O + 5-FAM-TSATLQSGFRK(QXL520)-NH2 <-> 5-FAM-TSATLQ + SGFRK(QXL520)-NH2

3CLpro + 8-Trifluoromethyladenine <-> Enzyme-inhibitor complex

H2O + Dabcyl-KNSTLQSGLRKE-Edans <-> SGLRKE-Edans + Dabcyl-KNSTLQ

H2O + MCA-AVLQSGFR-Lys(Dnp)-Lys-NH2 <-> SGFR-Lys(Dnp)-Lys-NH2 + MCA-AVLQ

H2O + 2-Aminobenzoyl-SVTLQSG-Tyr(NO2)-R <-> SG-Tyr(NO2)-R + 2-Aminobenzoyl-SVTLQ

H2O + (HiLyteFluor488)-ESATLQSGLRKAK-(QXL520)-NH2 <-> SGLRKAK-(QXL520)-NH2 + (HiLyteFluor488)-ESATLQ

H2O + NMA-TSAVLQSGFRK(DNP)-NH2 <-> SGFRK(DNP)-NH2 + NMA-TSAVLQ

H2O + ABZ-SAVLQSGFRK(DNP)-NH2 <-> SGFRK(DNP)-NH2 + ABZ-SAVLQ

H2O + ABZ-VLQSGFRK(DNP)-NH2 <-> SGFRK(DNP)-NH2 + ABZ-VLQ

H2O + ABZ-LQSGFRK(DNP)-NH2 <-> SGFRK(DNP)-NH2 + ABZ-LQ

H2O + ABZ-QTSITSAVLQSGFRK(DNP)-NH2 <-> SGFRK(DNP)-NH2 + ABZ-QTSITSAVLQ

H2O + ABZ-LYQPPQTSITSAVLQSGFRK(DNP)-NH2 <-> SGFRK(DNP)-NH2 + ABZ-LYQPPQTSITSAVLQ

H2O + ABZ-SGADVLYQPPQTSITSAVLQSGFRK(DNP)-NH2 <-> SGFRK(DNP)-NH2 + ABZ-SGADVLYQPPQTSITSAVLQ

H2O + ABZ-SAVLQSGK(DNP)-NH2 <-> ABZ-SAVLQ + SGK(DNP)-NH2

H2O + ABZ-SAVLQSGFRKMAK(DNP)-NH2 <-> ABZ-SAVLQ + SGFRKMAK(DNP)-NH2

H2O + ABZ-SAVLQSGFRKMAFPSGK(DNP)-NH2 <-> ABZ-SAVLQ + SGFRKMAFPSGK(DNP)-NH2

H2O + 2-Aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide <-> SGFRK-2,4-dinitrophenyl amide + 2-Aminobenzoyl-TSAVLQ

H2O + H-TSAVLQSGFRK-NH2 <-> H-SGFRK-NH2 + H-TSAVLQ-OH

H2O + SAVLQMGFRK <-> MGFRK + SAVLQ

H2O + (Ala-Arg-Leu-Gln-NH)2-Rhodamine <-> (Ala-Arg-Leu-Gln-NH) + (Ala-Arg-Leu-Gln-NH)-Rhodamine

H2O + SWTSAVLQSGFRKWA <-> SGFRKWA + SWTSAVLQ

H2O + (CAL Flour Red 610)-Thr-Ser-Ala-Val-Leu-Gln-Ser-Gly-Phe-Arg-(BHQ-1) <-> Ser-Gly-Phe-Arg-(BHQ-1) + (CAL Flour Red 610)-Thr-Ser-Ala-Val-Leu-Gln

H2O + ABZ-Thr-Ser-Ala-Val-Leu-Gln-Ser-Gly-Phe-Arg-Tyr(NO2)Gly-NH2 <-> Ser-Gly-Phe-Arg-Tyr(NO2)Gly-NH2 + ABZ-Thr-Ser-Ala-Val-Leu-Gln

H2O + SITSAVLQSGFRKMA <-> SGFRKMA + SITSAVLQ

H2O + VSYGSTLQAGLRKMA <-> AGLRKMA + VSYGSTLQ

H2O + H-KVATVQSKMSD-NH2 <-> H-SKMSD-NH2 + H-KVATVQ-OH

H2O + H-GPFVDRQTAQAAGTDT-NH2 <-> H-TAQAAGTDT-NH2 + H-GPFVDRQ-OH

H2O + Tamra-SITSAVLQSGFRKMAK-Dabcyl <-> SGFRKMAK-Dabcyl + Tamra-SITSAVLQ

H2O + SITSAVLQ-pNA <-> 4-Nitroaniline + SITSAVLQ

H2O + SITSAVLQ-pNP <-> p-Nitrophenol + SITSAVLQ

Quercetin 3-O-beta-D-galactoside + 3CLpro <-> Enzyme-inhibitor complex

H2O + Acetyl-Thr-Ser-Thr-Lys-Leu-Gln-7-amino-4-carbamoyl-coumarin <-> Acetyl-Thr-Ser-Thr-Lys-Leu-Gln + 7-Amino-4-carbamoyl-coumarin

External links

Expasy
KEGG
BRENDA
IntEnz (EBI)
IUBMB
Reactome
MetaCrop

View all entries for EC Class: 3.4.22.69

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