Enzyme Details

EC Classification

3.4.21.62

Enzyme name
(recommended)

subtilisin

Alternative names

alcalase
alcalase 0.6L
alcalase 2.5L
ALK-enzyme
bacillopeptidase A
bacillopeptidase B
Bacillus subtilis alkaline proteinase bioprase
bioprase AL 15
bioprase APL 30
colistinase
subtilisin J
subtilisin S41
subtilisin Sendai
subtilisin GX
subtilisin E
subtilisin BL
genenase I
esperase
maxatase
alcalase
thermoase PC 10
protease XXVII
thermoase
superase
subtilisin DY
subtilopeptidase
SP 266
savinase 8.0L
savinase 4.0T
kazusase
protease VIII
opticlean
Bacillus subtilis alkaline proteinase
protin A 3L
savinase
savinase 16.0L
savinase 32.0 L EX
orientase 10B
protease S

Classification

Hydrolases

Acting on peptide bonds (peptide hydrolases)

Serine endopeptidases

Catalyses reactions

H2O + n+m Peptide <-> n Peptide + m Peptide

H2O + Peptidyl-p-nitroanilide <-> Peptide + 4-Nitroaniline

H2O + Peptide-thiobenzyl ester <-> Peptide + Benzylthiol

H2O + Ac-LVSADNIDIS <-> NH2-NIDIS-OH + Ac-LVSAD

H2O + Ac-LVSAENIDIS <-> Ac-LVSAE + NH2-NIDIS-OH

H2O + Ac-LVSAANIDIS <-> Ac-LVSAA + NH2-NIDIS-OH

H2O + Ac-LASADNIDIS <-> NH2-NIDIS-OH + Ac-LASAD

H2O + Succinyl-Ala-Ala-Pro-Phe-p-nitroanilide <-> 4-Nitroaniline + Succinyl-Ala-Ala-Pro-Phe

H2O + Ac-Tyr-OEt <-> Ethanol + N-Acetyl-L-tyrosine

H2O + N-Benzoyl-L-tyrosine ethyl ester <-> Ethanol + N-Benzoyl-L-tyrosine

H2O + N-Acetyl-L-phenylalanine ethyl ester <-> Ethanol + N-Acetyl-L-phenylalanine

H2O + N-Benzoyl-L-arginine ethyl ester <-> Ethanol + N-Benzoyl-L-arginine

H2O + Hemoglobin <-> hydrolyzed Hemoglobin

H2O + Succinyl-Ala-Ala-Pro-Ala-p-nitroanilide <-> 4-Nitroaniline + Succinyl-Ala-Ala-Pro-Ala

H2O + Succinyl-Ala-Ala-Val-Ala-p-nitroanilide <-> 4-Nitroaniline + Succinyl-Ala-Ala-Val-Ala

H2O + Succinyl-Ala-Ala-Pro-Met-p-nitroanilide <-> 4-Nitroaniline + Succinyl-Ala-Ala-Pro-Met

H2O + Glp-Ala-Ala-Leu-p-Nitroanilide <-> 4-Nitroaniline + Glp-Ala-Ala-Leu

H2O + N-Acetyl-L-tryptophan methyl ester <-> Methanol + N-Acetyl-L-tryptophan

H2O + N-Acetyl-N-formyl-L-tryptophan methyl ester <-> Methanol + N-Acetyl-N-formyl-L-tryptophan

H2O + N-Acetyl-2-hydroxy-L-tryptophan methyl ester <-> Methanol + N-Acetyl-2-hydroxy-L-tryptophan

H2O + N-Acetyl-L-phenylalanine methyl ester <-> Methanol + N-Acetyl-L-phenylalanine

H2O + N-Acetyl-4-nitro-L-phenylalanine methyl ester <-> Methanol + N-Acetyl-4-nitro-L-phenylalanine

H2O + N-Acetyl-4-acetamido-L-phenylalanine methyl ester <-> Methanol + N-Acetyl-4-acetamido-L-phenylalanine

H2O + N-Acetyl-L-tyrosine methyl ester <-> Methanol + N-Acetyl-L-tyrosine

H2O + N-Acetyl-O-acetyl-L-tyrosine methyl ester <-> Methanol + N-Acetyl-O-acetyl-L-tyrosine

H2O + N-Acetyl-3-nitro-L-tyrosine methyl ester <-> Methanol + N-Acetyl-3-nitro-L-tyrosine

H2O + N-Acetyl-O-acetyl-3-nitro-L-tyrosine methyl ester <-> Methanol + N-Acetyl-O-acetyl-3-nitro-L-tyrosine

H2O + N-Acetyl-L-m-tyrosine methyl ester <-> Methanol + N-Acetyl-L-m-tyrosine

H2O + N-Acetyl-L-o-tyrosine methyl ester <-> Methanol + N-Acetyl-L-o-tyrosine

H2O + N-Acetyl-3,4,-dihydroxy-L-phenylalanine methyl ester <-> Methanol + N-Acetyl-3,4,-dihydroxy-L-phenylalanine

H2O + N-Acetyl-O-trans-p-phenylazobenzoyl-L-tyrosine methyl ester <-> Methanol + N-Acetyl-O-trans-p-phenylazobenzoyl-L-tyrosine

H2O + Benzyloxycarbonyl-Ala-Ala-Leu-p-nitroanilide <-> 4-Nitroaniline + Benzyloxycarbonyl-Ala-Ala-Leu

H2O + Benzyloxycarbonyl-Ala-Ala-Phe-p-nitroanilide <-> 4-Nitroaniline + Benzyloxycarbonyl-Ala-Ala-Phe

H2O + Benzyloxycarbonyl-Ala-Leu-p-nitroanilide <-> 4-Nitroaniline + Benzyloxycarbonyl-Ala-Leu

H2O + Benzyloxycarbonyl-Thr-Leu-p-nitroanilide <-> 4-Nitroaniline + Benzyloxycarbonyl-Thr-Leu

H2O + Benzyloxycarbonyl-Pro-Leu-p-nitroanilide <-> 4-Nitroaniline + Benzyloxycarbonyl-Pro-Leu

H2O + Benzyloxycarbonyl-Val-Leu-p-nitroanilide <-> 4-Nitroaniline + Benzyloxycarbonyl-Val-Leu

H2O + Benzyloxycarbonyl-Ala-Ala-Ala-p-nitroanilide <-> 4-Nitroaniline + Benzyloxycarbonyl-Ala-Ala-Ala

H2O + Benzyloxycarbonyl-Ala-Ala-Ala-p-nitroanilide <-> Ala-p-nitroanilide + Benzyloxycarbonyl-Ala-Ala

H2O + Benzyloxycarbonyl-Ala-Ala-Nva-p-nitroanilide <-> Nva-p-nitroanilide + Benzyloxycarbonyl-Ala-Ala

H2O + Benzyloxycarbonyl-Ala-Ala-Nva-p-nitroanilide <-> 4-Nitroaniline + Benzyloxycarbonyl-Ala-Ala-Nva

H2O + Benzyloxycarbonyl-Ala-Ala-Nle-p-nitroanilide <-> 4-Nitroaniline + Benzyloxycarbonyl-Ala-Ala-Nle

H2O + Benzyloxycarbonyl-Ala-Ala-Nle-p-nitroanilide <-> Nle-p-nitroanilide + Benzyloxycarbonyl-Ala-Ala

H2O + Benzyloxycarbonyl-Ala-Ala-p-nitroanilide <-> 4-Nitroaniline + Benzyloxycarbonyl-Ala-Ala

H2O + Benzyloxycarbonyl-Ala-Ala-p-nitroanilide <-> Ala-p-nitroanilide + Benzyloxycarbonyl-Ala

H2O + Benzyloxycarbonyl-Phe-Leu-p-nitroanilide <-> 4-Nitroaniline + Benzyloxycarbonyl-Phe-Leu

H2O + Benzyloxycarbonyl-Phe-Leu-p-nitroanilide <-> Leu-p-nitroanilide + Benzyloxycarbonyl-Phe

External links

Expasy
KEGG
BRENDA
IntEnz (EBI)
IUBMB
Reactome
MetaCrop

View all entries for EC Class: 3.4.21.62

© HITS gGmbH