Enzyme Details

 EC Classification 3.4.21.62
 Enzyme name
(recommended)
subtilisin
 Alternative names alcalase
alcalase 0.6L
alcalase 2.5L
ALK-enzyme
bacillopeptidase A
bacillopeptidase B
Bacillus subtilis alkaline proteinase bioprase
bioprase AL 15
bioprase APL 30
colistinase
(see also comments)
subtilisin J
subtilisin S41
subtilisin Sendai
subtilisin GX
subtilisin E
subtilisin BL
genenase I
esperase
maxatase
alcalase
thermoase PC 10
protease XXVII
thermoase
superase
subtilisin DY
subtilopeptidase
SP 266
savinase 8.0L
savinase 4.0T
kazusase
protease VIII
opticlean
Bacillus subtilis alkaline proteinase
protin A 3L
savinase
savinase 16.0L
savinase 32.0 L EX
orientase 10B
protease S
 Classification
Hydrolases
Acting on peptide bonds (peptide hydrolases)
Serine endopeptidases
 Catalyses reactions
H2O + n+m Peptide <-> n Peptide + m Peptide
H2O + Peptidyl-p-nitroanilide <-> Peptide + 4-Nitroaniline
H2O + Peptide-thiobenzyl ester <-> Peptide + Benzylthiol
H2O + Ac-LVSADNIDIS <-> NH2-NIDIS-OH + Ac-LVSAD
H2O + Ac-LVSAENIDIS <-> Ac-LVSAE + NH2-NIDIS-OH
H2O + Ac-LVSAANIDIS <-> Ac-LVSAA + NH2-NIDIS-OH
H2O + Ac-LASADNIDIS <-> NH2-NIDIS-OH + Ac-LASAD
H2O + Succinyl-Ala-Ala-Pro-Phe-p-nitroanilide <-> 4-Nitroaniline + Succinyl-Ala-Ala-Pro-Phe
H2O + Ac-Tyr-OEt <-> Ethanol + N-Acetyl-L-tyrosine
H2O + N-Benzoyl-L-tyrosine ethyl ester <-> Ethanol + N-Benzoyl-L-tyrosine
H2O + N-Acetyl-L-phenylalanine ethyl ester <-> Ethanol + N-Acetyl-L-phenylalanine
H2O + N-Benzoyl-L-arginine ethyl ester <-> Ethanol + N-Benzoyl-L-arginine
H2O + Hemoglobin <-> hydrolyzed Hemoglobin
H2O + Succinyl-Ala-Ala-Pro-Ala-p-nitroanilide <-> 4-Nitroaniline + Succinyl-Ala-Ala-Pro-Ala
H2O + Succinyl-Ala-Ala-Val-Ala-p-nitroanilide <-> 4-Nitroaniline + Succinyl-Ala-Ala-Val-Ala
H2O + Succinyl-Ala-Ala-Pro-Met-p-nitroanilide <-> 4-Nitroaniline + Succinyl-Ala-Ala-Pro-Met
H2O + Glp-Ala-Ala-Leu-p-Nitroanilide <-> 4-Nitroaniline + Glp-Ala-Ala-Leu
H2O + Glp-Ala-Ala-Leu-p-Nitroanilide <-> 4-Nitroaniline + Glp-Ala-Ala-Leu
H2O + N-Acetyl-L-tryptophan methyl ester <-> Methanol + N-Acetyl-L-tryptophan
H2O + N-Acetyl-N-formyl-L-tryptophan methyl ester <-> Methanol + N-Acetyl-N-formyl-L-tryptophan
H2O + N-Acetyl-2-hydroxy-L-tryptophan methyl ester <-> Methanol + N-Acetyl-2-hydroxy-L-tryptophan
H2O + N-Acetyl-L-phenylalanine methyl ester <-> Methanol + N-Acetyl-L-phenylalanine
H2O + N-Acetyl-4-nitro-L-phenylalanine methyl ester <-> Methanol + N-Acetyl-4-nitro-L-phenylalanine
H2O + N-Acetyl-4-acetamido-L-phenylalanine methyl ester <-> Methanol + N-Acetyl-4-acetamido-L-phenylalanine
H2O + N-Acetyl-L-tyrosine methyl ester <-> Methanol + N-Acetyl-L-tyrosine
H2O + N-Acetyl-O-acetyl-L-tyrosine methyl ester <-> Methanol + N-Acetyl-O-acetyl-L-tyrosine
H2O + N-Acetyl-3-nitro-L-tyrosine methyl ester <-> Methanol + N-Acetyl-3-nitro-L-tyrosine
H2O + N-Acetyl-O-acetyl-3-nitro-L-tyrosine methyl ester <-> Methanol + N-Acetyl-O-acetyl-3-nitro-L-tyrosine
H2O + N-Acetyl-L-m-tyrosine methyl ester <-> Methanol + N-Acetyl-L-m-tyrosine
H2O + N-Acetyl-L-o-tyrosine methyl ester <-> Methanol + N-Acetyl-L-o-tyrosine
H2O + N-Acetyl-3,4,-dihydroxy-L-phenylalanine methyl ester <-> Methanol + N-Acetyl-3,4,-dihydroxy-L-phenylalanine
H2O + N-Acetyl-O-trans-p-phenylazobenzoyl-L-tyrosine methyl ester <-> Methanol + N-Acetyl-O-trans-p-phenylazobenzoyl-L-tyrosine
H2O + Benzyloxycarbonyl-Ala-Ala-Leu-p-nitroanilide <-> 4-Nitroaniline + Benzyloxycarbonyl-Ala-Ala-Leu
H2O + Benzyloxycarbonyl-Ala-Ala-Phe-p-nitroanilide <-> 4-Nitroaniline + Benzyloxycarbonyl-Ala-Ala-Phe
H2O + Benzyloxycarbonyl-Ala-Leu-p-nitroanilide <-> 4-Nitroaniline + Benzyloxycarbonyl-Ala-Leu
H2O + Benzyloxycarbonyl-Thr-Leu-p-nitroanilide <-> 4-Nitroaniline + Benzyloxycarbonyl-Thr-Leu
H2O + Benzyloxycarbonyl-Pro-Leu-p-nitroanilide <-> 4-Nitroaniline + Benzyloxycarbonyl-Pro-Leu
H2O + Benzyloxycarbonyl-Val-Leu-p-nitroanilide <-> 4-Nitroaniline + Benzyloxycarbonyl-Val-Leu
H2O + Benzyloxycarbonyl-Ala-Ala-Ala-p-nitroanilide <-> 4-Nitroaniline + Benzyloxycarbonyl-Ala-Ala-Ala
H2O + Benzyloxycarbonyl-Ala-Ala-Ala-p-nitroanilide <-> Ala-p-nitroanilide + Benzyloxycarbonyl-Ala-Ala
H2O + Benzyloxycarbonyl-Ala-Ala-Nva-p-nitroanilide <-> Nva-p-nitroanilide + Benzyloxycarbonyl-Ala-Ala
H2O + Benzyloxycarbonyl-Ala-Ala-Nva-p-nitroanilide <-> 4-Nitroaniline + Benzyloxycarbonyl-Ala-Ala-Nva
H2O + Benzyloxycarbonyl-Ala-Ala-Nle-p-nitroanilide <-> 4-Nitroaniline + Benzyloxycarbonyl-Ala-Ala-Nle
H2O + Benzyloxycarbonyl-Ala-Ala-Nle-p-nitroanilide <-> Nle-p-nitroanilide + Benzyloxycarbonyl-Ala-Ala
H2O + Benzyloxycarbonyl-Ala-Ala-p-nitroanilide <-> 4-Nitroaniline + Benzyloxycarbonyl-Ala-Ala
H2O + Benzyloxycarbonyl-Ala-Ala-p-nitroanilide <-> Ala-p-nitroanilide + Benzyloxycarbonyl-Ala
H2O + Benzyloxycarbonyl-Phe-Leu-p-nitroanilide <-> 4-Nitroaniline + Benzyloxycarbonyl-Phe-Leu
H2O + Benzyloxycarbonyl-Phe-Leu-p-nitroanilide <-> Leu-p-nitroanilide + Benzyloxycarbonyl-Phe
 External links Expasy
KEGG
BRENDA
IntEnz (EBI)
IUBMB
Reactome
MetaCrop


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