Enzyme Details

EC Classification

1.14.15.6

Enzyme name
(recommended)

cholesterol monooxygenase (side-chain-cleaving)

Alternative names

cholesterol,reduced-adrenal-ferredoxin:oxygen oxidoreductase (side-chain-cleaving)
cholesterol desmolase
cytochrome P-450scc
desmolase, steroid 20-22
C27-side chain cleavage enzyme
cholesterol 20-22-desmolase
cholesterol C20-22 desmolase
cholesterol side-chain cleavage enzyme
cholesterol side-chain-cleaving enzyme
enzymes, cholesterol side-chain-cleaving
steroid 20-22 desmolase
steroid 20-22-lyase

Classification

Oxidoreductases

Acting on paired donors with incorporation of molecular oxygen

With a reduced iron-sulfur protein as one donor, and incorporation of one atom of oxygen

Catalyses reactions

H+ + NADPH + O2 + Cholesterol <-> H2O + NADP+ + 20alpha-Hydroxycholesterol

H+ + NADPH + O2 + Cholesterol <-> H2O + NADP+ + 22beta-Hydroxycholesterol

O2 + Cholesterol + Reduced adrenal ferredoxin <-> H2O + Oxidized adrenal ferredoxin + 4-Methylpentanal + Pregnenolone

O2 + Ferredoxin + 20alpha,22beta-Dihydroxycholesterol <-> Ferredoxin + 4-Methylpentanal + Pregnenolone

H+ + NADPH + O2 + 17alpha,20alpha-Dihydroxycholesterol <-> H2O + NADP+ + 4-Methylpentanal + 17alpha-Hydroxypregnenolone

H+ + NADPH + O2 + 20alpha-Hydroxycholesterol <-> H2O + NADP+ + 20alpha,22beta-Dihydroxycholesterol

H+ + NADPH + O2 + 22beta-Hydroxycholesterol <-> H2O + NADP+ + 20alpha,22beta-Dihydroxycholesterol

O2 + Reduced adrenal ferredoxin + Cholesta-5,7-dien-3beta-ol <-> H2O + Oxidized adrenal ferredoxin + 4-Methylpentanal + 7-Dehydropregnenolone

O2 + Reduced ferredoxin + Cholest-5-ene-3beta,25-diol <-> H2O + Oxidized ferredoxin + Pregnenolone + 4-Methyl-4-hydroxypentanal

External links

Expasy
KEGG
BRENDA
IntEnz (EBI)
IUBMB
Reactome
MetaCrop

View all entries for EC Class: 1.14.15.6