Enzyme Details

EC Classification

1.14.14.25

Enzyme name
(recommended)

cholesterol 24-hydroxylase

Alternative names

cholesterol,NADPH:oxygen oxidoreductase (24-hydroxylating)
cholesterol 24-monooxygenase
CYP46
CYP46A1
cholesterol 24S-hydroxylase
cytochrome P450 46A1

Classification

Oxidoreductases

Acting on paired donors with incorporation of molecular oxygen

With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen

Catalyses reactions

H+ + NADPH + O2 + Cholesterol <-> H2O + NADP+ + 24(S)-Hydroxycholesterol

H+ + NADPH + O2 + 24(S)-Hydroxycholesterol <-> H2O + NADP+ + Dihydroxycholesterol

H+ + NADPH + O2 + Bufuralol <-> H2O + NADP+ + 1-Hydroxy bufuralol

H+ + NADPH + O2 + Progesterone <-> H2O + NADP+ + 17alpha-Hydroxyprogesterone

H+ + NADPH + O2 + Dextromethorphan <-> H2O + NADP+ + 3-Methoxydextrorphanin

H+ + NADPH + O2 + Dextromethorphan <-> H2O + NADP+ + Dextrorphan

H+ + NADPH + O2 + Cortisol <-> Unknown + H2O + NADP+

H+ + NADPH + O2 + Diclofenac <-> H2O + NADP+ + 4'-Hydroxydiclofenac

H+ + NADPH + O2 + Phenacetin <-> H2O + Ethanol + NADP+ + Acetaminophen

H+ + NADPH + O2 + Testosterone <-> H2O + NADP+ + 6beta-Hydroxytestosterone

H+ + NADPH + O2 + Testosterone <-> H2O + NADP+ + 2-Hydroxytestosterone

H+ + NADPH + O2 + Testosterone <-> H2O + NADP+ + 16beta-Hydroxytestosterone

External links

Expasy
KEGG
BRENDA
IntEnz (EBI)
IUBMB
Reactome
MetaCrop

View all entries for EC Class: 1.14.14.25